C. Dale Poulter
John A. Widtsoe Distinguished Professor and Chair
B.S., Louisiana State University, 1964
Office: 2274 HEB-N
Activities & Awards
- Nakanishi Prize, American Chemical Society
- James Flack Norris Award, American Chemical Society
- Repligen Award, Biological Division, American Chemical Society
- Ernest Guenther Award, American Chemical Society
- Arthur C. Cope Scholar Award, American Chemical Society
- Alfred P. Sloan Fellow
- National Institute of Health Research Career Development Award
- Rosenblatt Prize, University of Utah
- David P. Gardner Fellow
- Distinguished Research Award, University of Utah
- Utah Award, American Chemical Society
- The Governor's Medal for Science and Technology
- Editor-in-Chief, Journal of Organic Chemistry
- Executive Committee, Biological Division of the American Chemical Society
- Fellow, American Academy of Arts and Sciences
My research group is interested in problems at the interface between organic chemistry and biochemistry. We study reactions catalyzed by enzymes in the isoprene biosynthetic pathway with special emphasis on establishing the mechanisms of the enzyme-catalyzed transformations and how the enzymes promote the reactions.
We also study structure-function relationships in peptides and proteins to determine how the particular topology of a complex biological molecule is related to its function as a catalyst or a ligand for receptor binding.
The isoprene biosynthetic pathway is needed by all organisms to produce essential compounds. We are studying several key enzymes in the pathway that catalyze fundamental reactions, including the isomerization of isopentenyl diphosphate (IPP) to dimethylallyl diphosphate (DMAPP), the condensation of IPP with a variety of allylic diphosphates to yield new allylic isoprenylogues containing five additional carbons, the unusual rearrangement of presqualence diphosphate (PSPP) to squalene during biosynthesis of cholesterol, and the posttranslational modifications of proteins by isoprenoid units that are important in cell signalling events. We are also studying unusual isoprenoid alkylations that occur during biosynthesis of ergot alkaloids, post-translational modifications of proteins, and in transfer RNAs.
We are isolating genes for the enzymes we study, construct plasmids for overexpression of the enzymes, and carrying out side-directed mutagenesis on critical amino acids to elucidate their role in catalysis. All of the reactions presented above are interesting biological alkylations in that they do not rely on commonly observed carbonyl group chemistry for construction of carbon-carbon and carbon--heteroatom bonds.Work in this area provides training in a combination of biochemical (purification of enzymes, kinetics, precursor-product studies) and chemical (synthesis, isolation-identification, reaction mechanisms) techniques.
Much of what we do relies heavily on modern analytical methods such as high pressure liquid chromatography, high field multinuclear magnetic resonance spectroscopy, and gas chromatography-mass spectrometry. We draw on ideas and techniques from organic chemistry and biochemistry to design our experiments, including developing new procedures for synthesis of compounds, developing new methods for measuring interactions between an enzyme and its substrates, and using recombinant DNA technology to obtain biological molecules that are normally difficult to isolate. It is the interdisciplinary nature of our work that we find both challenging and exciting.
- Walker, J.R.; Rothman, S.C.; Poulter, C.D. Synthesis and Evaluation of Substrate Analogues as Mechanism Based Inhibitors of Type II Isopentenyl Diphosphate Isomerase. J. Org. Chem. 2008, 73, 726-729.
- Thulasiram, H.V.; Erickson, H.K.; Poulter, C.D. A Common Mechanism for Branching, Cyclopropanation, and Cyclobutanation Reactions in the Isoprenoid Biosynthetic Pathway. J. Am. Chem. Soc. 2008, 130, 1966-1971.
- McDougal, O. M.; Turner, M.W.; Ormond, A.J.; Poulter, C.D. Three-Dimensional Structure of Conotoxin tx3a: An m-1 Branch Peptide of the M-Superfamily. Biochemistry 2008, 47, 2826-2832.
- Rothman, S.C.; Johnston, J.B.; Lee, S.; Walker, J.R.; Poulter, C.D. Type II Isopentenyl Diphosphate Isomerase: Irreversible Inactivation by Covalent Modification of Flavin. J. Am. Chem. Soc. 2008, 130, 4906-4913.
- Lee, S.; Poulter, C.D. Cloning, Solubilization, and Characterization of Squalene Synthase from Thermosynechococcus elongates BP-1. J. Bacteriol. 2008, 190, 3808-3816.
- de Ruyck, J.; Pouyez, J.; Rothman, S.C.; Poulter, C.D.; Wouters, J. Crystal Structure of Type 2 Isopentenyl Diphosphate Isomerase from Thermus thermophilus in Complex with Inorganic Pyrophosphate. Biochemistry 2008, 47, 9051-9053.
- Poulter, C.D.; Bioorganic Chemistry. A Natural Reunion of the Physical and Life Sciences. J. Org. Chem. 2009, 74, 2631-2645.
- Pan, J.-J.; Bugni, T.S.; Poulter, C.D. Recombinant Squalene Synthase. Synthesis of Cyclopentyl Non-Head-to-Tail Triterpenes. J. Org. Chem. 2009, 74, 7562-7565.
- Chen, M.; Poulter, C.D. Characterization of Thermophilic Archaeal Isopentenyl Phosphate Kinases., Biochemistry 2010, 49, 207-217.
- Mabanglo, M.F.; Schubert, H.L.; Chen, M.; Hill, C.P.; Poulter, C.D. X-ray Structures of Isopentenyl Phosphate Kinase. ACS Chem. Biol. 2010, 5, 317-327.
- Koohang, A.; Bailey, J.L.; Coates, R.M.; Erickson, H.L.; Owen, D.; Poulter, C.D. Enantioselective Inhibition of Squalene Synthase by Azridine Analogues of Presqualane Diphosphate. J. Org. Chem. 2010, 75, 4769-4777.
- Sharma, N.K.; Pan, J.J; Poulter, C. D. Type II Isopentenyl Diphosphate Isomerase: Probing the Mechanism with Alkyne/Allene Diphosphate Substrate Analogues. Biochemistry 2010, 49, 6228-6233.
- Poulter, C.D.; Hahn, F.; Cornish, R.; Testa, C. Antibacterial and Herbicidal Compounds and System for Screening the Same. U.S. Patent 7,736,881, June 15, 2010.
- Heaps, N. A.; Poulter, C. D. Synthesis and Evaluation of Chlorinated Substrate Analogues for Franesyl Diphosphate Synthase. J. Org. Chem 2011, 76, 1838-1843.