Directory: Faculty
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Jennifer S. Shumaker-ParryANALYTICAL CHEMISTRYAssistant Professor B.S. University of South Dakota, 1995 Phone: (801) 585-1434 Office: 2504 HEB-S Email: shumaker-parry@chem.utah.edu |
Research Interests
Molecular recognition plays a central role in biology by controlling cellular processes such as enzymatic catalysis, transport, regulation and communication. We are developing and applying high-throughput sensing methods based on surface plasmon resonance (SPR) to study molecular recognition between biomolecules. SPR-based sensing provides real-time, quantitative analysis of biomolecule interactions without the need for labels. Detailed information about the strength and specificity of biomolecule interactions impacts medical research, diagnostics, drug discovery and fundamental molecular biology studies. This multi-disciplinary research requires methods and tools from analytical chemistry, surface chemistry, biochemistry, materials science, nano\microfabrication, and microscopy.
Localized Surface Plasmon Resonance (LSPR) Sensing with Nanoparticles. We are developing novel nanoparticles as specific and sensitive nano-sized sensors. Metallic nanoparticle sensors are based on measuring changes in the LSPR when target molecules bind to surface-immobilized receptors. We are tailoring nanoparticle optical properties and developing methods for multiplexing, label-free analysis.
Cartoon of (a) a single nanoparticle sensor and (b) spectra (i.e., light scattering or extinction) for monitoring binding events.
Irregularly-Shaped Nanoparticles. One approach for tailoring nanoparticle properties is to control the physical structure. Noble metal nanoparticles exhibit plasmon resonances that depend on their size, shape and local environment. We are fabricating irregularly-shaped (i.e., non-spherical) nanostructures that exhibit unique plasmon resonances and localized field enhancements.
We are characterizing the nanoparticles using scanning electron microscopy (SEM), atomic force microscopy (AFM), UV-visible spectroscopy and dark field microscopy. Irregularly-shaped nanoparticles have potential application in bioanalytical chemistry, including surface-enhanced spectroscopies (e.g., Surface-Enhanced Raman Spectroscopy (SERS), Surface-Enhanced Infrared Spectroscopy, and Plasmon-Enhanced Fluorescence Spectroscopy).
SPR Microscopy for Array-Based Analysis of Biomolecule Interactions. SPR microscopy is emerging as an important technique for array-based molecular recognition studies. SPR microscopy provides a label- free method for high-throughput, quantitative, real-time kinetic studies of biomolecule interactions (e.g., protein-DNA, protein-protein, protein-vesicle). We are using SPR microscopy for array-based analysis and optimizing sensor surface chemistry and immobilization of binding partners (e.g., proteins or DNAs) for array fabrication.
Selected Publications
- Sardar, R.; Bjorge, N.S.; Shumaker-Parry, J.S., "Asymmetrically Functionalized Gold Nanoparticles Organized in One-Dimensional Chains," Nano Letters2008, 8, 731-736.
- Sardar, R.; Park, J.-W., Shumaker-Parry, J.S., “Facile Single-Step Aqueous Phase Synthesis and Stabilization of Gold and Silver Nanoparticles,” Langmuir 2007, 23, 11883-11889.
- Sardar, R.; Heap, T.; Shumaker-Parry, J.S., “Versatile Solid Phase Synthesis of Gold Nanoparticle Dimers Using an Asymmetric Functionalization Approach,” J. Am. Chem. Soc. 2007, 129, 5356-5357.
- Bukasov, R. and Shumaker-Parry, J.S., “Highly-Tunable Infrared Extinction Properties of Gold Nanocrescents,” Nano Lett. 2007, 7, 1113-1118.
- "Fabrication of Crescent-Shaped Optical Antennas," J.S. Shumaker-Parry, H. Rochholz, M. Kreiter, Adv. Mater. 2005, 17, 2131-2134.
- "Quantitative Methods for Spatially-Resolved Adsorption/Desorption Measurements in Real Time by SPR Microscopy," J.S. Shumaker-Parry, C.T. Campbell, Anal. Chem. 2004, 76, 907-917.
- "Microspotting Streptavidin and Double-Stranded DNA Arrays on Gold for High-Throughput Studies of Protein-DNA Interactions by SPR Microscopy," J.S. Shumaker-Parry, H. Zareie, R. Aebersold, C.T. Campbell, Anal. Chem. 2004, 76, 918-929.
- "Parallel, Quantitative Measurement of Protein Binding to a 120-Element Double-Stranded DNA Array in Real Time Using SPR Microscopy," J.S. Shumaker-Parry, R. Aebersold, C.T. Campbell, Anal. Chem. 2004, 76 , 2071.
- "A Streptavidin Linker Layer That Functions After Drying," N. Xia, J.S. Shumaker-Parry, M.H. Zareie, C.T. Campbell, D.G. Castner, Langmuir 2004, 20 , 3710.
- "Quantification of Tight Binding to Surface-Immobilized Phospholipid Vesicles Using Surface Plasmon Resonance: Binding Constant of Phospholipase A2," L.S. Jung, J.S. Shumaker-Parry, S.S. Yee, C.T. Campbell, M.H. Gelb, J. Am. Chem. Soc.2000, 122 , 4177-4184.