Professor Dale Poulter

Professor C. Dale Poulter

Education

  • B.S., 1964, Louisiana State University (Cum Laude)
  • Ph.D., 1967, University of California at Berkeley, with W.G. Dauben
  • Postdoctoral, 1967-69, University of California at Los Angeles, with S. Winstein

Contact

Office: HEB 2274

Phone: 801.581.6685

Email:poulter@chem.utah.edu

Awards

  • Honorary Professor, University of Queensland, Brisbande, Australia, 2002-2004
  • Repligen Award, Biological Division, American Chemical Society, 2002
  • Wilsmore Fellow, Melbourne University, 2001
  • Rosenblatt Award, University of Utah, 1999
  • Arthur C. Cope Scholar Award, American Chemical Society, 1998
  • Governor's Medal for Science and Technology, 1995
  • National Institutes of Health Merit Award, 1995
  • Fellow, American Association for the Advancement of Science, 1994
  • Utah Award, American Chemical Society, 1992
  • Ernest Guenther Award, American Chemical Society, 1991
  • Distinguished Research Award, University of Utah, 1984
  • David P. Gardner Faculty Fellow, 1983
  • Alfred P. Sloan Fellow, 1975-79
  • NIH Career Development Awardee, 1975-80
  • NIH Postdoctoral Fellow, 1967-69
  • NIH Predoctoral Fellow, 1965-67
  • Omnicron Delta Kappa, 1964
  • Phi Lambda Upsilon, 1964
  • Phi Kappa Phi, 1964

Professional Positions

  • Distinguished Professor of Chemistry, University of Utah, 1996-present
  • Chair, Department of Chemistry, University of Utah, 1995-2000
  • John A. Widtsoe Professor of Chemistry, University of Utah, 1994-present
  • Research Professor, Department of Medicinal Chemistry, University of Utah, 1978-present
  • Adjunct Professor, Department of Biochemistry, University of Utah, 1986-present
  • Professor, Department of Chemistry, University of Utah, 1978-1994
  • Director, Interdepartmental Program in Biological Chemistry, 1990-1993
  • Associate Professor, Department of Chemistry, University of Utah, 1975-1978
  • Associate Research Professor, Department of Biopharmaceutical Sciences, University of Utah, 1975-1978
  • Assistant Professor, Department of Chemistry, University of Utah, 1969-1975
  • Assistant Research Professor, Department of Biopharmaceutical Sciences, University of Utah, 1971-1975
  • Chemist, Esso Research Laboratory, Baton Rouge, Louisiana, Summer 1964
  • Chemist, PPG Chemicals, Lake Charles, Louisiana, Summers, 1962, 1963

Professional Activities

  • NAS, Bio2010, Chemistry sub-committee, 2001
  • AAAS, Member-at-Large, 2001-present
  • CS, Advisory Committee on Graduate Education, 2000-present
  • ACS, Committee on Professional Training, 1996-present
  • ACS, Councilor, Biological Division, 1993-present
  • AAAS, Nominating Committee Chemistry Division, 1997-1999, Chair 1999
  • ACS, Chair of Organic Division, 1998
  • ACS, Selection Committee; Editor of Organic Letters, 1998
  • ACS, Committee on Science, 1996-1999
  • NSF, Workshop on Organic Synthesis and Natural Products, co-organizer, 1993-1995
  • 14th Enzyme Mechanisms Conference, Scottsdale, AZ, Chair, 1993-95
  • 13th Enzyme Mechanisms Conference, Key Largo, FL, organizing committee, 1991-97
  • NIH, General Medical Sciences Council, 1990
  • ACS, American Coordinator, Biological Program, Pacific Basin Chemical Congress, 1989, 1995
  • PRF, Advisory Board, 1988-1990
  • ACS, Selection Committee; Editor of Journal of Organic Chemistry, 1988
  • ACS, Program Chairman for Biological Division, 1987
  • ACS, Canvassing Committee, Roger Adams Award in Organic Chemistry, 1986-1990
  • Gordon Conference on Enzymes, Coenzymes, and Metabolic Pathways, Chair, 1985
  • ACS, Executive Committee Organic Division, 1983-1986
  • NAS, Workshop on Effort Reporting Requirements of OMB Circular A-21; 1983
  • NIH, Ad Hoc member and Special Study Sections; 1980-1993
  • 7th Enzyme Mechanisms Conference; Sanibel, Florida; organizing committee, 1981-1983
  • ACS, Ad Hoc Task Force to monitor the Journal of Organic Chemistry; 1980-1981
  • NIH, Medicinal Chemistry A Study Section; 1977-81

Editorial Activities

  • Editor-in-Chief, Journal of Organic Chemistry, 2001- present
  • Senior Editor, Journal of Organic Chemistry, 2000-2001
  • Associate Editor, Organic Letters, 1999-2001
  • Associate Editor, Journal of Organic Chemistry, 1990-1995
  • Editorial Advisory Board, Chemical Reviews, 1996-2001
  • Editorial Advisory Board, Bioorganic & Medicinal Chemistry, 1993-present
  • Editorial Advisory Board, Journal of Organic Chemistry, 1987-1990
  • Editorial Board, Journal of Bioorganic Chemistry, 1983-1990
  • Editorial Advisory Board, Journal of the American Chemical Society, 1983-1986

Publications

  • Koppisch, A.T.; Blagg, B.S.J.; Poulter, C.D. "Synthesis of 2-C-Methyl-D-erythritol-4-phosphate: The First Pathway Specific Intermediate in the Methylerythritol Phosphate Route to Isoprenoids", Org. Lett., 2000, 2, 215-217.
  • Radisky, E.S.; Poulter, C.D. "Squalene Synthase: Steady State, Pre-Steady State, and Isotope Trapping Studies", Biochemistry, 2000, 39, 1748-1760.
  • Dolence, J.M.; Steward, L.E.; Dolence, E.K.; Wong, D.H. Poulter, C.D. "Overproduction of the Yeast CaaX Prenyl Protease Rce1p in Saccharomyces cerevisiae and Its Initial Biochemical Characterization", Biochemistry, 2000, 39, 4096-4104.
  • Harris, C.M.; Poulter, C.D. "Recent Studies of the Mechanism of Protein Prenylation", Nat. Prod. Rept., 2000, 17, 131-214.
  • Soderberg, T.; Poulter, C.D. "Escherichia coli Dimethylallyl Diphosphate:tRNA Dimethylallyltransferase: Essential Elements for Recognition of tRNA Substrates Within the Anticodon Stem-Loop", Biochemistry,, 2000, 39, 6546-6553.
  • Trueblood, C.E.; Boyartchuk, V.L.; Picologlou, E.; Rozema, D.; Poulter, C.D.; Rine, J. "The CaaX Proteases, Afc1p and Rce1p, Have Overlapping but Distinct Substrate Specificities", Molec. Cell. Biol., 2000, 20.
  • Moore, J.A.; Mathis, J.R.; Poulter, C.D. "Escherichia coli Dimethylallyl Diphosphate: tRNA Dimethylallyltransferase: Presteady State Kinetic Studies," Biochim et Biophys Acta, 2000, 1479, 166-174.
  • Phan, R.M.; Poulter, C.D. "Synthesis of Geranyl S-thiolodiphosphate. A New Alternate Substrate/Inhibitor for Prenyltransferases", Org Lett, 2000, 2, 2287-2289.
  • Dolence, E.K.; Dolence, J.M.; Poulter, C.D. "Solid-Phase Synthesis of a Farnesylated CaaX Peptide Library: Inhibitors of the Ras CaaX Endoprotease", J. Comb. Chem, 2000 2,522-536.
  • Fernandez, S.M.; Kellogg, B.A.; Poulter, C.D. "Farnesyl Diphosphate Synthase. Altering the Catalytic Site to Select for Geranyl Diphosphate Activity", Biochemistry, 2000, 39, 15316-17.
  • Hahn, F.M.; Eubanks, L.M.; Blagg, B.S.J.; Testa, C.; Baker, J.A..; Poulter, C.D. "1-Deoxyxylulose-5-Phosphate Synthase: The Gene Product of both ORF 2816 and ORF 2895 in Rhodobacter capsulatus", J. Bacteriology, 2001, 183, 1-11.
  • Dunford, J.E.; Coxon, F.P.; Luckman, S.P.; Thompson, K.; Hahn, F.M.; Poulter, C.D.; Rogers, M.J. "Identification of FPP Synthase, the Molecular Target of Nitrogen-Containing Bisphosphonates, Using a Cell-Free Protein Prenyl Assay", J. Pharm. Exper. Therapeutics, 2001, 296, 235-242.
  • Dolence, E.K.; Dolence, J.M.; Poulter, C.D. "Solid-Phase Synthesis of a Radiolabeled, Biotinylated, and Farnesylated Ca1a2X Peptide Substratea for Ras- and a-Mating Factor Converting Enzyme", Bioconjugate Chem., 2001, 12, 35-43.
  • Soderberg, T.; Poulter, C.D. "Escherichia coli Dimethylallyl Diphosphate:tRNA Dimethylallyltransferase: Site-Directed Mutagenesis of Highly conserved Residues", Biochemistry, 2001, 40, 1734-1740.
  • Rivera, S.B.; Swedlund, B.D.; King, G.J.; Bell, R.N.; Hussey, Jr., C.E.; Shattuck-Eidens, D.M.; Wrobel, W.M.; Peiser, G.D.; Poulter, C.D. "Chrysanthemyl Diphosphate Synthase: Isolation of the Gene and Characterization of the Recombinant Non-Head-to-Tail Monoterpene Synthase from Chrysanthemum cinerariaefolium", Proc. Natl. Acad. Sci. US, 2001, 98, 4373-4378.
  • Phan, R.M.; Poulter, C.D. "Synthesis of (S)-Isoprenoid Thiodiphosphates as Substrates and Inhibitors", J. Org. Chem., 2001, 66, 6705-6710.
  • Soderberg, T.; Chen, A.; Poulter, C.D. "Geranylgeranylglyceryl Phosphate Synthase. Characterization of the Recombinant Enzyme from Methanobacterium thermoautotrophicum", Biochemistry, 2001, 40,14847-14854.
  • Mu, Y.Q.; Eubanks, L.M.; Poulter, C.D.; Gibbs, R.A. "Coupling of Isoprenoid Triflates with Organoboron Nucleophiles: Synthesis and Biological Evaluation of Geranylgeranyl Diphosphate Analogs" Bioorg. Med. Chem., 2002, 10, 1207-1219.
  • Koppish, A.T.; Fox, D.T.; Blagg, B.S.J.; Poulter C.D. "E. coli MEP Synthase: Steady-State Kinetic Analysis and Substrate Binding" Biochemistry, 2002, 41, 236-243.
  • Fox, D.T.; Poulter, C.D. "Synthesis of (E)-4-Hydroxydimethylallyl Diphosphate. An Intermediate in the Methyl Erythritol Phosphate Branch of the Isoprenoid Pathway" J. Org. Chem., 2002, 67, 5009-5010.
  • Jarsfter, M.B.; Zhang, D.L.; Poulter, C.D. "Recombinant Squalene Synthase. Synthesis of Non-Head-to-Tail Isoprenoids in the Absence of NADPH" J. Am. Chem. Soc., 2002, 124, 8834-8845.
  • Blagg, B.S.J.; Jarstfer, M.B.; Rogers, D.H., Poulter, C.D. "Recombinant Squalene Synthase. A Mechanism for the Rearrangement of Presqualene Diphosphate to Squalene" J. Am. Chem. Soc., 2002, 124, 8846-8853.
  • Koppish, A.T.; Poulter, C.D. "Synthesis of 4-Diphosphocytidyl-2-C-methyl-D-erythritol and 2-C-Methyl-D-erythritol 4-Phosphate" J. Org. Chem., 2002, 67, 5416-5418.
  • Harris, C.M.; Derdowski, A.M.; Poulter, C.D. "Modulation of the Zn(II) Center in Protein Farnesyltransferase by Mutagenesis of the Zn(II) Ligands", Biochemistry, 2002, 41, 10554-10562.
  • Berezovski, M.; Li, W.-P.; Poulter, C.D.; Krylov, S. N. "Measuring the Activity of Farnesyltransferase by Capillary Electrophoresis with Laser-Induced Fluorescence Detection" Electrophoresis, 2002, 23, 3398-3403.
  • Iwata-Reuyl, D.; Math, S.K.; Poulter, C.D. "Bacterial Phytoene Synthase: Molecular Cloning, Over-Expression and Characterization of Erwinia herbicola Phytoene Synthase" Biochemistry, 2003, 42, 3359-3365.
  • Wouters, J.; Oudjama, Y.; Barkley, S.J.; Tricot, C.; Stalon, V.; Droogmans, L.; Poulter, C.D. "Catalytic Mechanism of E. coli Isopentenyl Diphosphate Isomerase Involves Cys67, Glu116 and Tyr104 as Suggested by Crystal Structures of Complexes with Transition State Analogues and Irreversible Inhibitors" J. Biol. Chem., 2003, 278, 11903-11908.
  • Eubanks, L.M.; Poulter, C.D. "Rhodobacter capsulatus 1-Deoxy-D-xylulose 5-Phosphate Synthase: Steady-State Kinetics and Substrate Binding" Biochemistry, 2003, 42, 1140-1149.
  • Hemmerlin, A.; Rivera, S.B.; Erickson, H.; Poulter, C.D. "Enzymes Encoded by the Farnesyl Diphosphate Synthase Gene Family in the Big Sagebrush Artemisia tridentata spp. spiciformis" J. Biol. Chem., 2003, 34, 32132-32140.
  • Erickson, H.; Poulter, C.D. "Chrysanthemyl Diphosphate Synthase. The Relationship among Chain Elongation, Branching and Cyclopropanation Reactions in the Isoprenoid Biosynthetic Pathway" J. Am. Chem. Soc., 2003, 23, 6885-6888.
  • Carrigan, C.N.; Poulter, C.D. "Zinc is an Essential Cofactor for Type I Isopentenyl Diphosphate: Dimethylallyl Diphosphate Isomerase" J. Am. Chem. Soc., 2003, 30, 9008-9009.
  • Barkley, S.J.; Cornish, R.M.; Poulter, C.D. "Identification of an Archaeal Type II Isopentenyl Diphosphate Isomerase in Methanothermobacter thermautotrophicus" J. Bacteriology, in press.
  • Testa, C.A.; Cornish, R.M.; Poulter, C.D. " The Sorbitol Phosphotransferase System is Responsible for the Transport of 2-C-Methyl D-Erythriotol into Salmonella enterica terovar Typhimurium" J. Bacteriology, 2004, 186, 473-480.
  • Scholte, A.; Eubanks, L.; Poulter, C.D.; Vederas, J. " Synthesis and Biological Activity of Isopentenyl Diphosphate Analogs." Bioorganic and Medical Chemistry, 2004, 12, 763-770.
  • Wouters, J; Oudjama, Y.; Stalon, V.; Droogmans, L.; Poulter, C. D. "Crystal Structure of the C67A Mutant of Isopentenyl Diphosphate Isomerase Complexed with a Mechanism-Based Irreversible Inhibitor" Proteins, 2004, 54, 216-221.
  • McDougal, O.M.; Poulter, C.D. "Three-Dimensional Structure of the Mini-M Conotoxin mr3a" Biochemistry, 2004, 43, 425-429.
  • Barkley, S.J; Desai, S. B.; Poulter, C.D. "Proton Exchange in Type II Isopentenyl Diphosphate Isomerase" Organic Letters, 2004, 6, 5019-5021.
  • Barkley, S.J.; Desai, S. B.; and Poulter, C.D. "Type II Isopentenyl Diphosphate Isomerase from Synechocystis sp. Strain PCC 6803"J. of Bacteriology, 2004, 8156-8158.
  • Fox, D.T.; Poulter, C.D. "Synthesis and Evaluation of 1-Deoxy-D-xylulose 5-Phosphoric Acid Analogs as Alternate Substrates for Methylerythritol Phosphate Synthase" J. Org. Chem., 2005, 70, 1978-1985.
  • Fox, D.T.; Poulter, C.D. "Mechanistic Studies with 2C-Methyl-D-erythritol 4-Phosphate Synthase from Escherichia coli" Biochemistry, 2005, 44, 8360-8368.
  • de Ruyck, J.; Rothman, S.C.; Poulter, C.D.; Wouters, J. "Structure of Thermus thermophilus type 2 isopentenyl diphosphates isomerase inferred from crystallography and molecular dynamics" Biochem. and Biophys. Res. Comm., 2005, 338, 1515-1518.
  • Walker, J.R.; Poulter, C.D. "Synthesis and Evaluation of 1-Deoxy-D-xylulose 5-Phosphate Analogs as Chelation Based Inhibitors of Methylerythritol Phosphate Synthase" J. Org. Chem., 2005, 70, 9955-9959.
  • Wu, Z.; Wouters, J.; Poulter, C.D. "Isopentenyl Diphosphate Isomerase. Mechanism-Based Inhibition by Diene Analogues of Isopentenyl Diphosphate and Dimethylallyl Diphosphte" J. Am. Chem. Soc., 2005, 127, 17433-17438.
  • Poulter, C.D. "Farnesyl Diphosphate Synthase. A Paradigm for Understanding Structure and Function Relationships in E-Polyprenyl Diphosphate Synthases" Phytochem. Reviews, 2006, 5, 17-26.
  • Testa, C.A.; Lherbet, C.; Pojer, F.; Noel, J.P; Poulter, C.D. "Cloning and Expression of IspDF from Mesorhizobium loti. Characterization of a Bifunctional Protein that Catalyzes Non-Consecutive Steps in the Methylerythritol Phosphate Pathway" Biochem. Biophys. Acta, 2006, 1764, 85-96.
  • Cornish, R.M.; Roth, J.R.; Poulter, C.D. "Lethal mutations in the isoprenoid pathway of Salmonella enterica" J. Bacteriol., 2006, 188, 1444-1450.
  • Lherbet, C.; Pojer, F.; Richard, S.B.; Noel, J.P.; Poulter, C.D. "Absence of Substrate Channeling between Active Sites in the Agrobacterium tumefaciens IspDF and IspE Enzymes of the Methyl Erythritol Phosphate Pathway" Biochemistry, 2006, 45, 3548-3553.
  • Thulasiram, H.V.; Phan, R.M.; Rivera, S.B.; Poulter, C.D. "Synthesis of Deuterium Labeled Derivatives of Dimethylallyl Diphosphate" J. Org. Chem., 2006, 71, 1739-1741.
  • Gauchet, C.; Labadie, G.R.; Poulter, C.D. "Regio- and Chemoselective Covalent Immobilization of Proteins through Unnatural Amino Acids" J. Am. Chem. Soc. 2006, 128, 9274-9275.
  • Lee, S.; Poulter, C.D. "Escherichia coli Type I Isopentenyl Diphosphate Isomerase: Structural and Catalytic Roles for Divalent Metals" J. Am. Chem. Soc. 2006, 128, 11545-11550.
  • Thulasiram, H.V.; Poulter, C.D. "Farnesyl Diphosphate Synthase: The Art of Compromise between Substrate Selectivity and Stereoselectivity" J. Am. Chem. Soc. 2006, 128, 15819-15823.
  • Rothman, S.C.; Poulter, C.D. "Kinetic and Spectroscopic Characterization of Type II Isopentenyl Diphosphate Isomerase from Thermus thermophilus: Evidence for Formation of Substrate-induced Flavin Species" Biochemistry, in press.
  • Thulasiram, H.V.; Erickson, H.K.; Poulter, C.D. "Chimeras of Two Isoprenoid Synthases Catalyze all Four Coupling Reactions in Isoprenoid Biosynthesis" Science 2007,316, 73-76.
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